Composite

Part:BBa_K2622029

Designed by: Auksė Gaižauskaitė   Group: iGEM18_Vilnius-Lithuania   (2018-10-10)


Lpp-OmpA-GFPN

Lpp-OmpA-GFPN has a GFP nanobody (BBa_K2622005) attached to Lpp-OmpA's (BBa_K1991004) C-terminus via glycine/serine linker, so that it could be displayed and detected in the outer surface of the membrane by binding to GFP. Lpp has no signal sequence as this part was designed to be used in vitro systems (liposomes, nanodiscs). This part should be expressed in IVTT's or bacteria lysates based on a T7 RNR polymerase, since it has T7 promoter and terminator.

This composite part has a RFC10 prefix and suffix, though it has an incompatible XbaI site between T7 promoter and RBS that must be avoided.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 39
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 456
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 39
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 39
    Illegal NgoMIV site found at 411
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 20


[edit]
Categories
Parameters
None